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AN EFFICIENT OXYGEN INDEPENDENT TWO‐PHOTON PHOTOSENSITIZATION MECHANISM
Author(s) -
SMITH G.,
McGimpsey W. G.,
LYNCH M. C.,
KOCHEVAR I. E.,
REDMOND R. W.
Publication year - 1994
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1994.tb05012.x
Subject(s) - rose bengal , trypan blue , photobleaching , flash photolysis , oxygen , photochemistry , chemistry , irradiation , absorption (acoustics) , biophysics , laser , fluorescence , materials science , biochemistry , cell , optics , biology , kinetics , organic chemistry , physics , quantum mechanics , nuclear physics , reaction rate constant , composite material
A novel oxygen‐independent photosensitization mechanism from the upper triplet state (T n ) of rose bengal has been demonstrated by selectively populating T n by sequential two‐color laser excitation. Products formed from T n inhibit red blood cell acetylcholinesterase and decrease viability of P388D 1 mouse macrophage monocyte cells as measured by trypan blue exclusion assay. Laser flash photolysis studies indicate that T n reacts efficiently, as evidenced by permanent photobleaching of T 1 absorption, with chemical yields approaching unit efficiency. This mechanism may have application for oxygen deficient photosensitization under high intensity, pulsed laser irradiation.