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PHOTOSENSITIZATION WITH A CHLORIN‐THIOBARBITURATE CONJUGATE
Author(s) -
Kessel David,
Morgan Alan
Publication year - 1994
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1994.tb02981.x
Subject(s) - absorbance , chlorin , conjugate , phototoxicity , photochemistry , fluorescence , chemistry , photodynamic therapy , tetrapyrrole , wavelength , irradiation , photosensitizer , materials science , chromatography , optics , organic chemistry , optoelectronics , in vitro , biochemistry , mathematical analysis , physics , mathematics , nuclear physics , enzyme
— The photobiologic properties of a chlorin‐thiobarbiturate conjugate were examined. In addition to the characteristic 670 nm chlorin absorbance, the conjugate exhibited new absorbance bands at 517 and 743 nm, but these were not a part of the fluorescence emission spectra, nor was fluorescence observed at wavelengths >670 nm. The action spectrum indicated that the long‐wavelength absorbance of the conjugate was not involved in phototoxicity in cell culture, and that only irradiation at wavelengths associated with the chlorin moiety yielded a cytotoxic effect. In vivo cytotoxicity elicited by 745 nm irradiation is attributed to thermal effects.