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ULTRAVIOLET‐INDUCED DNA DAMAGE AND ITS PHOTOREPAIR IN TAIL FIN CELLS OF THE MEDAKA, Oryzias lutipes
Author(s) -
Funayama Tomoo,
Mitani Hiroshi,
Shima Akihiro
Publication year - 1993
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1993.tb09578.x
Subject(s) - oryzias , endonuclease , irradiation , fin , biophysics , ultraviolet , dna , dna damage , in vivo , ultraviolet irradiation , ultraviolet light , microbiology and biotechnology , 3t3 cells , biology , chemistry , cell culture , materials science , photochemistry , biochemistry , genetics , transfection , physics , optoelectronics , gene , nuclear physics , composite material
— To study UV‐induced damage and its photorepair in vivo , endonuclease‐sensitive sites (ESS) induced by UV irradiation in the DNA of the tail fin of the Medaka, Oryzias latipes . were examined. Isolated fins were irradiated with UV‐B, but no ESS were detected after increasing doses of UV‐B up to 500 J/m 2 . Then fins were irradiated with UV‐B and fin cells were dissociated by treatment with trypsin and EDTA. Ultraviolet‐B‐induced ESS were detected in the epithelial cells near the tissue surface but not in the inner cells. The capacity for photo repair of both inner and outer epithelial cells and cells in fin ray was the same and was higher than that for a line of cultured cells, OL32.