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A CHARACTERIZATION OF THE FLUORESCENT PROPERTIES OF CIRCULATING HUMAN EOSINOPHILS
Author(s) -
Barnes Dan,
Aggarwal Shanti,
Thomsen Sharon,
Fitzmaurlce Maryann,
RichardsKortum Rebecca
Publication year - 1993
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1993.tb09565.x
Subject(s) - characterization (materials science) , fluorescence , chemistry , biophysics , biology , nanotechnology , materials science , physics , optics
— This paper presents a characterization of the fluorescence properties of human eosinophils isolated from peripheral blood of normal donors over a wide range of excitation and emission wavelengths. Circulating eosinophils possess three fluorescence excitation emission maxima: one at 280 nm excitation, 330 nm emission, attributable to tryptophan fluorescence, and currently unassigned peaks at 360 nm excitation, 440 nm emission and 380 nm excitation, 415 nm emission. Fluorescence microscopy studies show that the Huorescence of eosinophils may be site dependent; specifically, when observed at 365 nm excitation, circulating eosinophil Huorescence appears blue‐violet, while the fluorescence of tissue‐dwelling eosinophils appears amber‐gold. These results should be considered in developing an optical biopsy technique to identify eosinophils in human tissue.