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ISOLATION AND CHARACTERIZATION OF THE PRESUMED PHOTORECEPTOR PROTEIN OF Blepharisma japonicum
Author(s) -
Gioffré Domenico,
Ghetti Francesco,
Lenci Francesco,
Paradiso Cristina,
Dai Renke,
Song PillSoon
Publication year - 1993
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1993.tb09561.x
Subject(s) - isolation (microbiology) , characterization (materials science) , biology , microbiology and biotechnology , optics , physics
— Photosensory responses in the ciliated protozoan Blepharisma japonicum are mediated by a hypericin‐like chromophore, blepharismin, localized in granules distributed under the cell membrane. A blepharismin‐binding protein, with an apparcnt molecular weight ranging between 35 and 38 kDa, has been isolated by means of column separations and preparative isoelectric focusing and characterized by means of gel electrophoresis, analytic isoelectric focusing as well as absorption and fluorescence spectroscopy.