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LOW‐TEMPERATURE LUMINESCENCE SPECTRA AND FLUORESCENCE LIFETIMES OF POLYCYTIDYLIC ACID IN POLYALCOHOLIC GLASSES
Author(s) -
Večeř Jaroslav,
Heřman Petr,
Beránek Josef
Publication year - 1993
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1993.tb09212.x
Subject(s) - protonation , fluorescence , chemistry , luminescence , excimer , photochemistry , emission spectrum , monomer , ethylene glycol , polynucleotide , spectral line , analytical chemistry (journal) , materials science , organic chemistry , polymer , ion , optics , biochemistry , physics , optoelectronics , astronomy
— Corrected emission spectra and fluorescence lifetimes of polycytidylic acid in ethylene glycol: water glass at low temperatures are reported. Luminescence properties observed exhibit a strong dependence on pH and temperature. At neutral pH a vibronic structure of a blue part of the fluorescence spectrum is revealed when temperature is changed from 77 to 10 K, confirming that a monomer component of fluorescence is present. There is also a strong difference in decay of a red‐shifted excimer fluorescence at 10 K at pH 7 and pH 3.9, reflecting a different protonation of cytosine residues and different conformations of polynucleotides in such conditions.