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RECONSTITUTION OF ALLOPHYCOCYANIN FROM Mastigocladus laminosus WITH ISOLATED LINKER POLYPEPTIDE
Author(s) -
Gottschalk L.,
Lottspeich F.,
Scheer H.
Publication year - 1993
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1993.tb04966.x
Subject(s) - allophycocyanin , linker , circular dichroism , chemistry , fluorescence , ultracentrifuge , biochemistry , stereochemistry , crystallography , biophysics , cyanobacteria , biology , phycocyanin , genetics , physics , quantum mechanics , bacteria , computer science , operating system
The core linker polypeptide L c 8.9 was isolated from Mastigocladus laminosus and purified on a preparative scale. A method for the reconstitution of allophycocyanin (AP)—linker complexes from isolated polypeptides was developed. The complex (α AP (β AP ) 3 L c 8.9 was reconstituted and compared to (α AP β AP ) and (α AP β AP ) 3 by sucrose density gradient ultracentrifugation, absorption, fluorescence emission and circular dichroism spectroscopy. Differences in the spectra of reconstituted and of directly isolated AP complexes are discussed.