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DETECTION OF THE EXCITED SINGLET STATE OF A DEPROTONATED, REDUCED FLAVIN
Author(s) -
Heelis Paul F.,
Hartman Rosemarie F.,
Rose Seth D.
Publication year - 1993
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1993.tb02970.x
Subject(s) - flash photolysis , flavin group , deprotonation , photochemistry , excited state , chemistry , singlet state , ultrafast laser spectroscopy , singlet fission , aqueous solution , triplet state , photodissociation , laser , atomic physics , kinetics , reaction rate constant , physics , ion , organic chemistry , optics , quantum mechanics , enzyme
— The excited singlet state of a deprotonated, reduced flavin [1, 5‐dihydro‐ N (3)‐carboxymethyllumiflavin] in aqueous solution at pH 8 has been detected by laser flash photolysis. The broad absorption band maximized at ∼ 490 nm (ε= 9.9 × 10 3 M ‐1 cm ‐1 ). The lifetime of the transient was found to be 100 ± 15 ps. The lifetime was not affected by the presence of pyrimidine dimers, which would be monomerized under these conditions. A longer‐lived transient, tentatively identified as the solvated electron, was also detected. The neutral reduced flavin did not give a detectable transient.