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LASER‐INDUCED FLUORESCENCE IN MALIGNANT and NORMAL TISSUE OF RATS INJECTED WITH BENZOPORPHYRIN DERIVATIVE
Author(s) -
AnderssonEngels Stefan,
Ankerst Jaro,
Johansson Jonas,
Svanberg Katarina,
Svanberg Sune
Publication year - 1993
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1993.tb02958.x
Subject(s) - hematoporphyrin , fluorescence , chemistry , laser , derivative (finance) , phthalocyanine , photodynamic therapy , photosensitizer , laser induced fluorescence , biophysics , photochemistry , analytical chemistry (journal) , optics , biology , chromatography , physics , organic chemistry , financial economics , economics
— Laser‐induced fluorescence was used to characterize the localization of intravenously administered ben‐zoporphyrin derivative‐monoacid (BPD‐MA) 3 h postinjection in different rat tissue types, including an induced experimental malignant tumor. A comparison of the fluorescence properties and the demarcation potential between the newer sensitizer BPD‐MA and four other substances, hematoporphyrin (HP), polyhematoporphyrin ester (PHE), tetrasulfonated phthalocyanine (TSPc) and the commercially available Photofrin earlier investigated, is included. The fluorescence light was induced with a nitrogen laser, emitting at 337 nm. The fluorescence spectrum in the region380–750 nm was analyzed by a polychromator equipped with a diode array detector. The demarcation potential between tumor and surrounding tissue in terms of fluorescence signal for the tumor model used was 2:1 for BPD‐MA. In comparison with the other drugs, HP shows about the same demarcation potential, whereas Photofrin and PHE exhibit about 3 times better and TSPc about 1.5 times better demarcation. By also employing the endogenous tissue fluorescence signature the contrast was enhanced by a factor of about 2 for each of the five drugs.