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A PHYTOCHROME STUDY USING TWO‐LASER/TWO‐COLOR FLASH PHOTOLYSIS: I 700 IS A MANDATORY INTERMEDIATE IN THE P r P fr PHOTOTRANSFORMATION
Author(s) -
Scurlock Rodger D.,
Braslavsky Silvia E.,
Schaffner Kurt
Publication year - 1993
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1993.tb02939.x
Subject(s) - flash photolysis , laser , flash (photography) , chemistry , absorption (acoustics) , analytical chemistry (journal) , flash lamp , photochemistry , optics , chromatography , physics , kinetics , reaction rate constant , quantum mechanics
— The phototransformation of native (124 kDa)oat phytochrome, P r P fr , Has been studied at 10d̀C by two laser/ two‐color flash photolysis. the overall P r P fr reaction yield did not vary with temperature within the range4–21d̀C. Foloeing the excitation of P r with a single 15 ns laser flash at 650nm, the formation of P fr was quantitavely measured in a time‐resolved experiment in the presence of a second 8 ns laser flash at 710 nm delayed from the initial flash. the second laser flash causes at 1.0 s after the initial laser flash a depletion of the uintermediate I 700 as welll as a reduction of the P fr absorption at 730 nm. The depletion of I 700 correlates quantitavely with the reduction of P fr formation. The absorpton spectra of I 700 and of the following intermendiate, I bi , were calculated assuming that the amount of P r , which is photoconverted by a single laser, equals the amount of P fr formed.