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In vitro GRANULOMA FORMATION BY SPLEEN CELLS TREATED WITH PSORALEN PLUS LONG‐WAVE ULTRAVIOLET RADIATION
Author(s) -
Okamoto Hiroyuki,
Guo Zaipei,
Imamura Sadao
Publication year - 1993
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1993.tb02934.x
Subject(s) - trypan blue , spleen , in vitro , granuloma , psoralen , chemistry , pathology , granuloma formation , microbiology and biotechnology , immunology , biology , medicine , biochemistry , dna
— In vitro gransulomas ar induced by culturing murine spleen cells with artificial microparticles, dextran beads. In the presence of 0.5 μg/mL 8‐methoxypsorlen, UVA radiation (0.2‐2.0 J/cm 2 suppressed granuloma formatin in a UVA dose‐ dependent manner. The dose of PUVA did not affect the cell viability as assessed by trypan blue exclusion. The time course of granuloma formation in 0.5 J/cm 2 PUVA‐treated cells was similar to that of normal spleen cells, with a miximum granuloma index at day 3 of culture, althought a49–63% suppression of granuloma formation was observed in PUVA‐treated, adherent cells even when they were cultured with normal nonadherent cells. These data suggests that PUVA alters macrophages, resulting in the suppression of granuloma formation in vitro