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OSCILLATORY LOW‐LEVEL CHEMILUMINESCENCE FROM A NONEQUILIBRIUM β‐NICOTINAMIDE ADENINE DINUCLEOTIDE‐PEROXIDASE SYSTEM: EXPERIMENTAL OBSERVATIONS and COMPUTER SIMULATIONS
Author(s) -
Watanabe Nobuyuki,
Inaba Humio
Publication year - 1993
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1993.tb02335.x
Subject(s) - chemiluminescence , nicotinamide adenine dinucleotide , nad+ kinase , horseradish peroxidase , chemistry , nicotinamide , non equilibrium thermodynamics , oscillation (cell signaling) , peroxidase , cofactor , fluorescence , photochemistry , analytical chemistry (journal) , biophysics , enzyme , thermodynamics , chromatography , biochemistry , physics , optics , biology
Abstract Using a highly sensitive photon counting technique, oscillatory low‐level chemiluminescence was observed from a non‐equilibrium oxidation of β‐nicotinamide adenine dinucleotide (NADH) by horseradish peroxidase (HRP). Emergence and stability of the oscillation were affected by the concentration of HRP, 2,4‐dichlorophenol and infusion rate of NADH. We also constructed a simultaneous rate equation to explain the oscillatory phenomena of this non‐equilibrium system, in which the photon emission was assumed to be generated from NAD'‐O 2 interaction.