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LIPID PHASES IN RENAL BRUSH BORDER MEMBRANES REVEALED BY LAURDAN FLUORESCENCE *
Author(s) -
Levi Moshe,
Wilson Paul V.,
Cooper Otis J.,
Gratton Enrico
Publication year - 1993
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1993.tb02312.x
Subject(s) - laurdan , brush border , membrane , vesicle , chemistry , fluorescence anisotropy , fluorescence , membrane fluidity , membrane lipids , cholesterol , brush , biophysics , biological membrane , chromatography , biochemistry , materials science , biology , physics , quantum mechanics , composite material
Steady‐state fluorescence properties of 6‐dodecanoyl‐2‐dimethylaminonapththalene (Laurdan) have been used to determine the coexistence of separate lipid‐phase domains in apical brush border membrane vesicles isolated from the rat renal cortex. The temperature dependence of generated polarization has been utilized to quantitate the lipid phases. Finally, the effect of cholesterol enrichment on these parameters has been studied. The results indicate the coexistence of lipid‐phase domains in brush border membranes based on the values of the generalized polarization and assuming that the membrane state can be described by coexistence of gel and liquid crystalline state. At 37°C, approximately19–32% of the brush border membrane lipids are in the gel phase, and cholesterol enrichment causes a further concentration‐dependent increase in the gel phase of brush border membrane lipids.