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MODULATION AND DYNAMICS OF PHASE PROPERTIES IN PHOSPHOLIPID MIXTURES DETECTED BY LAURDAN FLUORESCENCE *
Author(s) -
Parasassi Tiziana,
Ravagnan Giampietro,
Rusch Ruth M.,
Gratton Enrico
Publication year - 1993
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1993.tb02309.x
Subject(s) - laurdan , phospholipid , vesicle , fluorescence , phase (matter) , chemistry , analytical chemistry (journal) , phosphatidylcholine , chemical physics , biophysics , membrane , chromatography , organic chemistry , optics , physics , biochemistry , biology
Steady‐state and dynamic fluorescence properties of 6‐lauroyl‐2‐dimethylaminonaphthalene (Laurdan) have been used to ascertain the coexistence of separate phase domains and their dynamic properties in phospholipid vesicles composed of different mole ratios of dilauroyl‐ and dipalmitoyl‐phosphatidylcholine (DLPC and DPPC, respectively). The recently introduced generalized polarization together with time‐resolved emission spectra have been utilized for detecting changes. The results indicate the coexistence of phospholipid phase domains in vesicle compositions in the range between 30 mol% and 70 mol% DPPC in DLPC. Below and above these concentrations a homogeneous phase is observed, with averaged properties. In the case of coexisting phase domains, the properties of each individual phase are largely influenced by the presence of the other phase. Implications on fluctuations between the coexisting phases and on the size and shape of domains are discussed.