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HYDROGEN PEROXIDE‐DEPENDENT GENERATION OF SINGLET MOLECULAR OXYGEN BY HUMAN SALIVA: ITS DETECTION BY CHEMILUMINESCENCE FROM A Cypridina LUCIFERIN ANALOG
Author(s) -
Takahama Umeo
Publication year - 1993
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1993.tb02304.x
Subject(s) - chemiluminescence , singlet oxygen , hydrogen peroxide , chemistry , luciferin , photochemistry , saliva , oxygen , peroxide , luminol , peroxidase , reactive oxygen species , light emission , organic chemistry , biochemistry , optics , luciferase , enzyme , transfection , physics , gene
By the addition of hydrogen peroxide to human saliva, chemiluminescence from Cypridina luciferin analog (CCLA) and oxygen evolution were observed. Chemiluminescence was inhibited by inhibitors of salivary peroxidase, azide and cyanide and by a singlet oxygen quencher, crocin. Deuterium oxide (99.75%) stimulated the initial increase of CCLA by15–50% and the integrated CCLA 2.1‐3.6‐fold. The result suggest that the generation of singlet oxygen by peroxidase in human saliva depends on hydrogen peroxide.