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FLUORESCENCE AND CIRCULAR DICHROISM STUDIES ON THE PHYCOERYTHROCYANINS FROM THE CYANOBACTERIUM
Author(s) -
Sai P. S. Maruthi,
Siebzehnrübl S.,
Mahajan S.,
SCHEER H.
Publication year - 1993
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1993.tb02257.x
Subject(s) - chromophore , circular dichroism , trimer , fluorescence , chemistry , photochemistry , phycobilisome , dissociation (chemistry) , magnetic circular dichroism , crystallography , dimer , spectral line , optics , cyanobacteria , physics , biology , organic chemistry , astronomy , bacteria , genetics
Two phycoerythrocyanin (PEC) fractions have been obtained from the phycobilisomes of the cyanobac‐terium Westiellopsis prolifica ARM 365. They have been characterized by absorption, fluorescence and circular dichroism spectroscopy. One of them is spectroscopically similar to a PEC trimer known from other organisms. Whereas efficient energy transfer from its violin (α‐84) to the cyanin (β‐84, 155) chromophores is efficient in the trimer (αβ it is impeded after dissociation to the monomer (α,β). A second fraction of PEC which we earlier termed PEC(X) (Maruthi Sai et al., Photochem. Photobiol. 55 ,119–124, 1992), exhibited the spectral properties similar to that of the α‐subunit of PEC from Mastigocladus laminosus. With this highly photoactive fraction, the circular dichroism spectra of the violobilin chromophore in both photoreversible states were obtained.