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PHOTOLYSIS OF RHODOPSIN RESULTS IN DEPROTONATION OF ITS RETINAL SCHIFF'S BASE PRIOR TO FORMATION OF METARHODOPSIN II
Author(s) -
Thorgeirsson Thorgeir E.,
Lewis James W.,
WallaceWilliams Stacie E.,
Kliger David S.
Publication year - 1992
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1992.tb09738.x
Subject(s) - deprotonation , schiff base , rhodopsin , microsecond , photochemistry , photodissociation , chemistry , absorption (acoustics) , absorption spectroscopy , retinal , decomposition , crystallography , optics , organic chemistry , physics , ion , biochemistry
— Absorption changes following photolysis of bovine rhodopsin in mildly sonicated membrane suspensions are monitored at 25°C. Difference spectra collected at 17 times between 1 μs and 75 ms following excitation are analyzed globally using singular value decomposition and non‐linear least‐squares fitting techniques. The results are not consistent with the simple scheme: Lumirhodopsin → Metarhodopsin I → Metarhodopsin II, but indicate that an intermediate with a deprotonated Schiff's base is formed nearly simultaneously with Metarhodopsin I upon the decay of Lumirhodopsin.