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PHOTOBLEACHING DIFFERENCE ABSORPTION SPECTRA OF HUMAN CONE PIGMENTS: QUANTITATIVE ANALYSIS AND COMPARISON TO OTHER METHODS
Author(s) -
Merbs Shannath L.,
Nathans Jeremy
Publication year - 1992
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1992.tb09708.x
Subject(s) - photobleaching , pigment , absorbance , absorption (acoustics) , chemistry , absorption spectroscopy , electroretinography , optics , spectral line , analytical chemistry (journal) , fluorescence , retinal , chromatography , biochemistry , physics , organic chemistry , astronomy
— Four human cone pigment apoproteins were expressed by transfection of human tissue culture cells with the corresponding complementary DNA clones. Following reconstitution of the cone pigments by incubation with 11 ‐cis retinal, photobleaching difference absorption spectra were obtained for the blue pigment, the green pigment, and two polymorphic variants of the red pigment. These spectra were analyzed to determine the wavelengths of maximal absorbance and the bandwidths. The recombinant cone pigment spectra were compared to human cone spectral sensitivities and cone pigment absorption spectra determined by microspectrophotometry, single‐cell electrophysiology, reflection densitometry, electroretinography, and psychophysical color and brightness matching.