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LIGHT‐INDUCED SYNTHESIS OF ANTHOCYANIN IN CARROT CELLS IN SUSPENSION—IV. THE ACTION SPECTRUM
Author(s) -
Takeda Junko,
Abe Shunnosuke
Publication year - 1992
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1992.tb09604.x
Subject(s) - action spectrum , fluence , anthocyanin , irradiation , wavelength , phytochrome , visible spectrum , biophysics , chemistry , suspension (topology) , absorption (acoustics) , optics , photochemistry , red light , botany , biology , physics , food science , nuclear physics , mathematics , homotopy , pure mathematics
— Using carrot cell suspension in 2,4‐dichlorophenoxyacetic acid (2,4‐D)‐depleted culture medium, fluence‐response curves for the formation of anthocyanin were determined at various wavelengths from 250 to 800 nm. In the fluence‐response curves at wavelengths between 260 and 330 nm, the response showed a sharp fluence‐dependent increase after the fluence exceeded threshold level at the respective wavelength. Such a sharp increase in response was not observed by light at 450 nm or longer wavelengths, although the response obtained by higher fluence of such light was always higher than that in the dark control. Action spectra determined at the sharp increasing phase of the response showed the single peak at 280 nm which equals the absorption maximum of UV‐B photoreceptor. Although red (R)‐light alone had a minor effect on anthocyanin accumulation, it modulated the action of UV‐B light. That is, when carrot cells were irradiated with R‐light either before or after UV‐B irradiation, anthocyanin formation was greatly enhanced above the level enhanced by UV‐B light alone. The most effective wavelength for this enhancement was 660 nm. The effect of R‐light on the anthocyanin formation of the UV‐B irradiated cells was reversed by immediately following it with far‐red light, suggesting the involvement of phytochrome in the R‐effect.