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EFFECT OF VITAMIN E ON CYTOTOXICITY, DNA SINGLE STRAND BREAKS, CHROMOSOMAL ABERRATIONS, AND MUTATION IN CHINESE HAMSTER V‐79 CELLS EXPOSED TO ULTRAVIOLET‐B LIGHT
Author(s) -
Sugiyama Masayasu,
Tsuzuki Katsuyuki,
Matsumoto Kumi,
Ogura Ryohei
Publication year - 1992
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1992.tb09598.x
Subject(s) - chinese hamster , cytotoxicity , genotoxicity , microbiology and biotechnology , dna damage , ultraviolet light , hamster , biology , dna , chemistry , biochemistry , in vitro , toxicity , photochemistry , organic chemistry
— The effect of pretreatment with vitamin E on cytotoxicity, DNA single strand breaks, and chromosomal aberrations as well as on mutation induced by ultraviolet‐B light (UV‐B) was investigated in Chinese hamster V‐79 cells. Cellular pretreatment with non‐toxic levels of 25 μM α‐tocopherol succinate (vitamin E) for 24 h prior to exposure resulted in a 10‐fold increase in cellular levels of α‐tocopherol. Using a colony‐forming assay, this pretreatment decreased the cytotoxicity of UV‐B light. However, alkaline elution assays demonstrated that pretreatment with vitamin E did not affect the number of DNA single strand breaks caused by UV‐B light. In addition, UV‐B exposure produced a dose‐dependent induction of chromosomal aberrations and mutations at the HGPRT locus, and neither of these actions of UV‐B was influenced by pretreatment with the vitamin. These results suggest that vitamin E protects cells from UV‐B‐induced cytotoxicity, possibly through its ability to scavenge free radicals. The results also suggest that the extent of genotoxicity induced by UV‐B light may not correlate directly with the cytotoxic action of this wavelength region in sunlight.