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DETERMINATION OF CYTOSINE‐CYTOSINE PHOTODIMERS IN THE DNA OF CLOUDMAN S91 MELANOMA CELLS USING HIGH PRESSURE LIQUID CHROMATOGRAPHY
Author(s) -
Niggli Hugo J.
Publication year - 1992
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1992.tb08526.x
Subject(s) - pyrimidine dimer , cytosine , thymine , cytidine , chemistry , dimer , dna , pyrimidine , irradiation , photochemistry , chromatography , dna damage , stereochemistry , biochemistry , organic chemistry , enzyme , physics , nuclear physics
— I measured the induction of cytosine‐cytosine dimer (C‐C) densities after UV‐C (< 290 nm) and UV‐B irradiation (290–320 nm) in the 2′‐deoxy‐[ 3 H]cytidine labeled DNA of Cloudman S91 mouse melanoma cells using a new, sensitive high pressure liquid chromatography procedure. UV‐B exposure resulted in 0.000034% C‐C/J m ‐2 of the total cytosine radioactivity which is 10 times less than the rate during UV‐C irradiation. Previous work with these melanoma cells showed a 4‐fold lower rate of induction of thymine‐containing pyrimidine dimers by UV‐B than UV‐C light (Niggli Photochem. Photobiol . 52 , 519–524, 1990). Based on these results, the calculated ratios for the pyrimidine dimer subspecies showed no significant difference following UV‐C and UV‐B exposure. However, UV‐C and UV‐B light induce 10–20 times more thymine‐containing pyrimidine dimers than C‐C in the DNA of S91 cells.