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EVALUATION OF PORPHYRIN CHARACTERISTICS REQUIRED FOR PHOTODYNAMIC THERAPY
Author(s) -
Woooburn K. W.,
Vardaxis N. J.,
Hill J. S.,
Kaye A. H.,
Reiss J. A.,
Phillips D. R.
Publication year - 1992
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1992.tb08513.x
Subject(s) - phototoxicity , porphyrin , photodynamic therapy , cationic polymerization , chemistry , partition coefficient , cytotoxicity , photochemistry , biophysics , chromatography , biochemistry , in vitro , polymer chemistry , organic chemistry , biology
— The cytotoxicity (in the dark), phototoxicity (red light) and subcellular localization (using confocal laser scanning microscopy) were determined for 15 porphyrins (1–15) in C 6 glioma cells. The partition coefficient in 2‐octanol was also determined for each porphyrin at pH 7.4. The cytotoxicity increased with π (log of partition coefficient) up to π values of +2. The 7 porphyrins with cationic side chains exhibited a classical parabolic correlation between phototoxicity and π, with maximal activity at a π value of approximately 1.0. There was also a significant correlation between subcellular localization and degree of phototoxicity, with the three most photosensitive porphyrins all possessing cationic side chains, and all three localizing in mitochondria.