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PARTIAL NUCLEOTIDE SEQUENCE OF PHYTOCHROME FROM THE ZYGNEMATOPHYCEAN GREEN ALGA Mougeotia *
Author(s) -
Winands Armin,
Wagner Gottfried,
Marx Stefan,
SchneiderPoetsch Hansjörg A. W.
Publication year - 1992
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1992.tb02232.x
Subject(s) - phytochrome , biology , intron , gene , nucleic acid sequence , genomic dna , microbiology and biotechnology , exon , genetics , biochemistry , botany , red light
— Following polymerase chain reaction, a fragment of about 800 bp was amplified from genomic Mougeotia DNA using oligonucleotides directed to conserved regions of known phytochrome genes. The nucleotide sequence points to a different exon/intron structure in the neighborhood of the chromophore attachment site of this Mougeotia phytochrome gene, as compared to other phytochromes. Alignment of the derived amino acid sequence to phytochromes of higher and lower plants shows highest homology (>60%) to type II (green type) phytochrome, while Northern blot analysis of total Mougeotia RNA indicates down‐regulation of the phytochrome transcription in light. Signal pattern of hybridized genomic DNA after digestion reveals the presence of probably only one phytochrome gene in Mougeotia .