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A FIBER‐OPTIC RATIO FLUOROMETER FOR MUTANT ISOLATION
Author(s) -
Horwitz Benjamin A.,
Amit Rivka,
Llpson Edward D.
Publication year - 1992
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1992.tb02181.x
Subject(s) - fluorometer , fluorescence , photobiology , chlorophyll fluorescence , mutant , photomorphogenesis , optics , chemistry , biophysics , biology , physics , arabidopsis , biochemistry , gene
— Mutants with altered fluorescence are likely to be modified in pigment composition or photochemistry. They can thus help address a variety of problems in photobiology, particularly in the areas of photosynthesis, photomorphogenesis and photobehavior. To aid in the screening for such mutants, we have developed an instrument for detecting fluorescence intensity relative to scattered light intensity. The instrument employs fiber optics for illumination and detection, and an analog electronic circuit to compute the ratio between fluorescence and scattered light. An application of the fluorometer to screen for Arabidopsis mutants altered in chlorophyll accumulation is described.