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THE PHOTOTOXICITY OF 8‐M‐ETHOXYTHIONEPSORALEN AND 6‐M‐ETHYLTHIONECOUMARIN
Author(s) -
Tuveson R. W.,
Wang GuiRong,
Becker Ralph S.
Publication year - 1992
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1992.tb02169.x
Subject(s) - phototoxicity , pbr322 , escherichia coli , photosensitizer , plasmid , dna , chemistry , microbiology and biotechnology , dna damage , recombinant dna , biology , photochemistry , biochemistry , in vitro , gene
— The phototoxicity of 8‐m‐ethoxythionepsoralen (8‐M‐O T P) and 6‐m‐ethylthione coumarin (6‐M‐ T C) when activated by UV‐A has been investigated using a variety of Escherichia coli strains, Haemophilus influenme transforming DNA and Escherichia coli pBR322 plasmid DNA. The results demonstrate that 8‐M‐O T P is a strictly oxygen independent photosensitizer that is about 500‐f‐old less efficient in forming lesions leading to equivalent lethality than is the parent compound from which it is derived (8‐M‐OP). As is true for 8MOP, 8‐M‐O T P is capable of inducing histidine independent mutations in E. coli and inactivating transforming DNA consistent with DNA being a target for lesions induced by this molecule in the presence of UV‐A. 6‐M‐ T C is a strongly oxygen dependent photosensitizer activated by UV‐A when tested with either E. coli cells or transforming DNA in contrast to the parent compound (6‐m‐ethylcoumarin; 6‐M‐C) which is not phototoxic when treated with UV‐A. These results imply that the membrane may be an important target leading to lethality. 6‐M‐TC in the presence of UV‐A can inactivate pBR322 plasmid and Haemophilus influenme transforming DNA activity in virro suggesting that DNA is a potential target for this molecule when activated by UV‐A.