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In vitro AND in vivo ULTRAVIOLET‐INDUCED ALTERATIONS OF OXY‐ AND DEOXYHEMOGLOBIN
Author(s) -
Kollia N.,
Baqer A.,
Sadiq I.,
Sayre R. M.
Publication year - 1992
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1992.tb02150.x
Subject(s) - methemoglobin , in vivo , ultraviolet , chemistry , in vitro , ultraviolet radiation , radiation , stoichiometry , hemoglobin , photochemistry , irradiation , biophysics , nuclear magnetic resonance , biochemistry , radiochemistry , materials science , biology , optics , optoelectronics , physics , genetics , nuclear physics
— Ultraviolet (UV) radiation was found to convert oxyhemoglobin and deoxyhemoglobin stoichiometrically into methemoglobin and a met‐like product, respectively. The peak conversion efficiency for oxyhemoglobin occurred at 285 nm and decreased by a factor of 100 by 315 nm. The peak conversion efficiency for deoxyhemoglobin occurred at 290 nm and decreased by a factor of 30 by 320 nm. The transformation of oxyhemoglobin to methemoglobin was also documented in intact erythrocytes using UV‐B radiation. Finally, similar transformations were found to occur in human skin with UV‐B exposure but not on all volunteers tested. These results imply that methemoglobin will be formed in vivo on solar exposure and provide evidence that UV‐B photons reach the blood vessels.