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ACTION SPECTRA OF PHTHALOCYANINES WITH RESPECT TO PHOTOSENSITIZATION OF CELLS
Author(s) -
Moan Johan,
Berg Kristian,
Bommer Jerry C.,
Western Andre
Publication year - 1992
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1992.tb02144.x
Subject(s) - action (physics) , chemistry , photochemistry , phthalocyanine , spectral line , physics , organic chemistry , quantum mechanics
— Human carcinoma cells (NHIK 3025 cells) and Chinese hamster cells (V79 cells) were incubated with AIPcS 1 , AIPcS 2 and AIPcS 4 , phthalocyanines with different lipophilicity but with similar photochemical properties when in monomeric solutions. The absorption‐ and fluorescence spectra of the dyes in the cells were recorded as well as their action spectra with respect to sensitizing cells to photoinactivation. These spectra show that under the present conditions AIPcS 1 is strongly aggregated in both cell lines; AIPcS 2 is aggregated in V79 cells but much less so in NHIK 3025 cells. A main finding is that the shapes of the action spectra are similar to that of the fluorescence excitation spectra, but not to the absorption spectra, indicating that the photosensitizing effects of the dyes are mainly due to their monomeric fraction in the cells. AIPcS 2 and AIPcS 4 localize intracellularly mainly in lysosomes while AIPcS 1 was found to be more diffusely distributed in cells. As measured per quantum of fluorescence emitted, AIPcS 2 and AIPcS 2 are more efficient sensitizers than AIPcS 4 . The difference in efficiency between AIPcS 2 and AIPcS 4 is supposedly due to a different localization pattern on the suborganelle level.