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A NEW FLUORESCENT CHOLESTEROL DERIVATIVE: STRUCTURE AND BLOOD PLASMA ANALYSIS
Author(s) -
Abubaker M. Amin,
Wandruszka Ray Von
Publication year - 1992
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1992.tb02143.x
Subject(s) - fluorescence , derivative (finance) , chemistry , cholesterol , plasma , biochemistry , physics , business , optics , quantum mechanics , finance
— A fluorescent cholesterol derivative produced by reaction with gaseous HCI and zinc chloride in ethyl acetate is shown to be a chlorine substituted B‐ring diene. The species forms relatively rapidly via an i‐steroid rearrangement, requiring a temperature around 70°C. A weak, possibly dimeric π‐allyl zinc complex exists in solution, leading to a red shift in the fluorescence emission. The application of the derivative to the determination of cholesterol in bovine plasma provides good sensitivity and precision and requires notably small sample volumes.