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EXPRESSION OF AP 3, 4 AND 5 ISOPHYTOCHROMES IN ETIOLATED OAT SEEDLINGS ( Avena sativa L.) *
Author(s) -
GRIMM RUDOLF,
ECKERSKORN CHRISTOPH
Publication year - 1991
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1991.tb08499.x
Subject(s) - etiolation , avena , asparagine , alanine , phytochrome , threonine , residue (chemistry) , amino acid , biochemistry , biology , chemistry , botany , serine , enzyme , red light
— Type 1 phytochrome from etiolated oat seedlings was digested with V‐8 protease. Microsequencing of a 13 kDa fragment yielded a sequence of 31 amino acids. The fragment starting with the alanine residue at position 427 of the entire phytochrome amino acid sequence revealed a heterogeneity (threonine, alanine and asparagine) at position 10. This demonstrates that the phytochrome type A genes AP3, 4 and 5 are expressed as proteins.