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INCREASED TRANSCRIPTION AND TRANSLATION OF HEME OXYGENASE IN CHINESE HAMSTER FIBROBLASTS FOLLOWING PHOTODYNAMIC STRESS OR PHOTOFRIN II INCUBATION
Author(s) -
GOMER CHARLES J.,
LUNA MARIAN,
FERRARIO ANGELA,
RUCKER NATALIE
Publication year - 1991
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1991.tb03934.x
Subject(s) - heme oxygenase , heme , hemin , chinese hamster , chemistry , oxygenase , microbiology and biotechnology , oxidative stress , transcription (linguistics) , downregulation and upregulation , hamster , blot , western blot , incubation , biochemistry , biology , gene , enzyme , in vitro , linguistics , philosophy
— Porphyrin mediated photosensitization can enhance the transcription and translation of several oxidative stress genes. In this study, we report on the enhanced expression of the gene encoding for heme oxygenase in Chinese hamster fibroblasts by; (1) incubation in Photofrin II; (2) Photofrin II mediated photosensitization; and (3) photosensitization induced by Rose Bengal. Increased expression of heme oxygenase mRNA was accompanied by a concomitant increase in the synthesis of the 34 kDa heme oxygenase protein. Western blot analysis using antibody to heme oxygenase confirmed the immunoreactivity of the 34 kDa protein induced by Photofrin II and PDT. These results demonstrate that heme oxygenase can be activated by non‐metalloporphyrins as well as by photosensitization associated with singlet oxygen mediated subcellular injury.