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PHOTOTOXICITY INVOLVING THE OCULAR LENS: in vivo AND in vitro STUDIES
Author(s) -
LERMAN SIDNEY,
MANDAL KRIS,
MISRA BIJAY,
SCHECHTER ANDREW,
SCHENCK JOHN
Publication year - 1991
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1991.tb03929.x
Subject(s) - lens (geology) , in vivo , phototoxicity , fluorescence , in vitro , chemistry , biophysics , nuclear magnetic resonance , optics , biology , biochemistry , physics , microbiology and biotechnology
— Our laboratory has demonstrated the cataractogenic potential of UV radiation and several photosensitizing drugs in laboratory animals and in humans. We have utilized lens fluorescence measurements (which we have demonstrated to be a reliable marker for pre‐cataractous and early cataractous changes), NMR pulse relaxation techniques, and our recently developed magnetic resonance imaging method to measure lens T 2 values in the normal and UV exposed Degus lens ( in vivo and in vitro ) to detect pre‐cataractous changes in the lens. These approaches will permit us to employ two parameters (increased non‐tryptophan fluorescence and a decrease in T 2 values) to monitor for such changes months before the lens opacities become manifest by conventional slit lamp examinations.