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FLUORESCENCE ENERGY TRANSFER BETWEEN DIMETHYLDIAZAPEROPYRENIUM DICATION AND ETHIDIUM INTERCALATED IN POLY d(A‐T)
Author(s) -
MERGNY JEANLOUIS,
SLAMASCHWOK ANNY,
MONTENAYGARESTIER THÉRÈSE,
ROUGÉE MICHEL,
HÉLÈNE CLAUDE
Publication year - 1991
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1991.tb03670.x
Subject(s) - ethidium bromide , polynucleotide , intercalation (chemistry) , fluorescence , chemistry , chromophore , dication , photochemistry , acceptor , excited state , energy transfer , dna , molecule , chemical physics , physics , optics , biochemistry , inorganic chemistry , organic chemistry , nuclear physics , condensed matter physics
— Dimethyldiazaperopyrenium is one of the largest known DNA intercalators. Fluorescence energy transfer occurred between dimethyldiazaperopyrenium (donor) and ethidium (acceptor) when these dyes were bound to a double‐stranded polynucleotide such as poly d(A‐T). The addition of increasing amounts of ethidium bromide led to a marked shortening of the fluorescence lifetime of the donor, whereas the excited state of the acceptor was progressively populated via energy transfer from the donor. Critical Förster distance between these two chromophores was calculated to be 3.8 nm. The observed transfer efficiency was lower than that calculated on the basis of this critical distance and a statistical distribution of bound drugs. These results are discussed taking into account the conformational change induced by intercalation of dimethyldiazaperopyrenium in the double‐stranded polynucleotide.