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α‐TERTHIENYL PHOTOSENSITIZES DAMAGE TO pBR322 DNA
Author(s) -
WANG TIAN P.,
KAGAN JACQUES,
TUVESON R. W.,
WANG G. R.
Publication year - 1991
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1991.tb03657.x
Subject(s) - pbr322 , dna damage , dna , chemistry , genetics , biology , plasmid
— α‐Terthienyl photosensitizes single strand breaks in pBR322 DNA. Almost identical results were observed under oxygen and under argon. In the presence of oxygen, this DNA nicking was enhanced by histidine and was not affected by superoxide dismutase, catalase, or the antioxidant BHT. Although chemical damage to DNA treated with α‐terthienyl plus near‐UV was clearly demonstrated in vitro , transformation in E. coli with this damaged pBR322 DNA still took place. Likewise, Haemophilus influenzae DNA transforming activity was not significantly decreased by photosensitization with α‐terthienyl.