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DYNAMIC LINEAR DICHROISM IN CHROMOPROTEINS
Author(s) -
LYLE PAUL A.,
STRUVE WALTER S.
Publication year - 1991
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1991.tb03641.x
Subject(s) - chromophore , linear dichroism , chemistry , fluorescence anisotropy , anisotropy , fluorescence , molecular physics , dichroism , excitation , perpendicular , chemical physics , acceptor , molecule , crystallography , optics , circular dichroism , photochemistry , physics , condensed matter physics , mathematics , organic chemistry , quantum mechanics , geometry
— The time‐dependent polarized fluorescence and optical density transients arising from electronic excitation transport are derived for solutions of randomly oriented chromoproteins in which the chromophore transition moments have well‐defined orientations with respect to the protein. The treatment is extended to oriented monolayers of chromoproteins, in which the molecules are aligned with a particular protein‐fixed axis perpendicular to the surface plane and with random azimuthal angles about the surface normal. The initial ratio of parallel to perpendicular transients is invariably 3:1, irrespective of system dimensionality and chromophore organization. The residual anisotropy observed at long times is directly related to the relative orientations of the donor and acceptor chromophores. These calculations form a basis for detailed interpretation of ultrafast fluoresence and pump‐probe linear dichroism studies in photosynthetic systems, for which 3‐dimensional structures are becoming increasingly available.