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QUANTUM YIELD OF CHAPSO‐SOLUBILIZED RHODOPSIN and 3‐HYDROXY RETINAL CONTAINING BOVINE OPSIN *
Author(s) -
Gärtner W.,
Ullrich D.,
Vogt K.
Publication year - 1991
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1991.tb02128.x
Subject(s) - rhodopsin , opsin , retinal , retinaldehyde , quantum yield , pigment , absorbance , chromophore , chemistry , photochemistry , biology , chromatography , biochemistry , fluorescence , organic chemistry , optics , physics
– The quantum yields of bleaching for two artificial pigments, bovine opsin combined with (3ft)‐3‐hydroxy retinal or (3 R ,S)‐3‐methoxy retinal, were determined in comparison to the value for regenerated bovine rhodopsin. Regeneration of the visual pigments was performed by incubation of 3‐[(3‐Cholamidopropyl)‐dimethylammonio]‐2‐hydroxy‐1‐propanesulfonate (CHAPSO)‐solubilized opsin with the 11‐ cis isomers of retinal and the respective retinal derivatives. The extinction coefficients of the pigments in CHAPSO were determined to 35000 M −1 cm −1 (native rhodopsin), 35300 M −1 cm −1 (regenerated rhodopsin) and 34500 M −1 cm −1 (3‐OH retinal opsin). With respect to rhodopsin (Λ max : 500 nm), the pigments carrying the substituted chromophores exhibit blue shifted absorbance maxima (3‐hydroxy and 3‐methoxy retinal opsin: 488 nm). In parallel experiments under absolutely identical conditions we find related to the value of CHAPSO solubilized rhodopsin (1) a quantum efficiency of bleaching for the 3‐hydroxy pigment of 1.2.