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BATHOPRODUCTS and CONFORMERS OF ALL‐trans‐ AND 13‐ds BACTERIORHODOPSIN AT 90 K *
Author(s) -
Balashov S. P.,
Karneyeva N. V.,
Litvin F. F.,
Ebrey T. G.
Publication year - 1991
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1991.tb02115.x
Subject(s) - bacteriorhodopsin , conformational isomerism , chemistry , absorption (acoustics) , photochemistry , halobacterium , absorption spectroscopy , reaction rate constant , spectral line , analytical chemistry (journal) , kinetics , materials science , molecule , optics , membrane , organic chemistry , physics , biochemistry , quantum mechanics , astronomy , composite material
– At 90 K the photoproduct of the primary light reaction of (rani‐bacteriorhodopsin, the bathoproduct K 1 consists of a mixture of at least three spectrally different species, K 1 I , K 1 II , and K 1 III having maxima in the difference absorption spectra at 645, 635 and 625 nm, respectively. The bathoproducts differ in their long wavelength absorption bands and in their rate constants for photo‐conversion to trans ‐bacteriorhodopsin under far red light irradiation (λ > 720 nm). The bathoproducts are formed from different precursors–conformers of trans ‐bacteriorhodopsin, which are stable at 90 K, but are in equilibrium with each other at 213 K. We suggest that the bathoproducts may initiate parallel conversion cycles of trans ‐bacteriorhodopsin at low temperatures. The primary photoreaction of 13‐ cis ‐bacteriorhodopsin also yields three bathoproducts, K c I , K c II and K c III having maxima in the difference absorption spectra at 615, 605 and 595 nm, respectively.