Premium
EFFECTS OF CHRONIC EXPOSURE ULTRAVIOLET‐A INCLUDING 2% ULTRAVIOLET‐B ON FREE RADICAL REDUCTION SYSTEMS IN HAIRLESS MICE
Author(s) -
Maeda Kazuhisa,
Naganuma Masako,
Fukuda Minoru
Publication year - 1991
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1991.tb02083.x
Subject(s) - hairless , elastin , superoxide dismutase , chemistry , connective tissue , hydroxyproline , glutathione , glutathione peroxidase , skin aging , antioxidant , irradiation , biochemistry , enzyme , pathology , dermatology , medicine , physics , nuclear physics
Chronic ultraviolet (UV) irradiation is known to cause a variety of changes in the skin, including wrinkles, pigmented spots and carcinogenesis. To explore time dependent changes in several parameters with chronic UV irradiation, we examined the molecular changes in connective tissue, intracellular defence enzymes and free radical antioxidant substances in hairless mice skin caused by chronic exposure to UV‐A including 2% UV‐B. Connective tissue changes were estimated using hydroxyproline and isodesmosine assays as a measure of collagen and elastin concentrations, respectively. After 6 weeks irradiation, the insoluble collagen and elastin were both substantially elevated, as were the activities of glutathione peroxidase (GSH‐Px) and superoxide dismutase (SOD). Continued UV irradiation resulted in a steady decline in SOD and lipid soluble antioxidants, while the GSH‐Px remained elevated, suggesting that SOD and lipid soluble antioxidants in the skin may be involved in protecting it from UV damage and deteriorate with chronic irradiation.