Premium
THE EFFECTS OF PLASMA LIPOPROTEINS ON in vitro TUMOR CELL KILLING and in vivo TUMOR PHOTOSENSITIZATION WITH BENZOPORPHYRIN DERIVATIVE
Author(s) -
Allison B. A.,
Waterfield E.,
Richter A. M.,
Levy J. G.
Publication year - 1991
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1991.tb02079.x
Subject(s) - in vivo , in vitro , tumor cells , chemistry , derivative (finance) , plasma lipoprotein , plasma , cell , cancer research , biophysics , biochemistry , biology , lipoprotein , cholesterol , physics , microbiology and biotechnology , quantum mechanics , financial economics , economics
The influence of lipoprotein association on in vitro tumor cell killing and in vivo tumor photosensitization with benzoporphyrin derivative (BPD) has been investigated in M‐1 tumor bearing mice. The association of benzoporphyrin mono acid ring A with either low or high density lipoprotein increased tumor cell killing in an in vivolin vitro cytotoxicity assay performed 3 h post intravenous drug administration. Eight hours following photosensitizer injection only low density lipoprotein (LDL) mixtures produced significant ( P ≤ 0.005) increases in tumor cell killing compared to BPD in unfractionated plasma. The efficacy of in vivo photosensitization in the presence of lipoproteins correlated with the in vivolin vitro cytotoxicity. Association of BPD with low or high density lipoproteins resulted in delayed tumor regrowth and higher cure rates when light exposure (125J/cm 2 ) was performed 3 h post drug administration. When light exposure was performed 8 h post‐injection only LDL‐BPD mixtures led to enhanced tumor eradication compared to BPD administered in aqueous solution or unfractionated plasma.