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ARTIFACTS IN FLUORESCENCE EMISSION SPECTROSCOPY RELATED TO WOOD'S ANOMALY
Author(s) -
Kessel David
Publication year - 1991
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1991.tb02045.x
Subject(s) - monochromator , fluorescence , polarizer , wavelength , grating , emission spectrum , optics , spectroscopy , fluorescence spectroscopy , materials science , spectral line , polarization (electrochemistry) , chemistry , physics , birefringence , quantum mechanics , astronomy
Fluorescence emission spectra of porphyrins acquired with a monochromator using a 1500 line/mm holographic grating were affected by polarization‐induced effects (Wood's anomaly) which affected the transmission of different wavelengths of light. This effect resulted in a blue‐shift in the emission optimum, and distortions in the fluorescence spectrum at longer wavelengths. These anomalies could be eliminated by insertion of polarizers, orientation = 0°, in emission and excitation paths. A monochromator with a 1200 line/mm holographic grating was essentially free from these distortions.