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NON‐DIMER DNA DAMAGE IN CHINESE HAMSTER V‐79 CELLS EXPOSED TO ULTRAVIOLET‐B LIGHT
Author(s) -
Matsumoto Kumi,
Sugiyama Masayasu,
Ogura Ryohei
Publication year - 1991
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1991.tb02032.x
Subject(s) - dna damage , chinese hamster , cytotoxicity , dna , ultraviolet light , dimer , microbiology and biotechnology , hamster , chemistry , incubation , ultraviolet , pyrimidine dimer , biology , biochemistry , in vitro , photochemistry , materials science , optoelectronics , organic chemistry
— To understand and characterize non‐dimer DNA damage and cytotoxicity induced by ultraviolet‐B light (UV‐B,290–320 nm), an alkaline elution technique for analysis of DNA damage was used on Chinese hamster V‐79 cells. Ultraviolet‐B exposure produced a dose‐dependent induction of DNA single strand breaks and DNA‐protein crosslinks; however, there was an absence of DNA‐DNA interstrand crosslinks. Neither of these types of DNA damage were repaired within a 24 h incubation of the cells following a single UV‐B exposure; rather the damage increased. Using a colony forming assay, we found that UV‐B exposure resulted in an increase of cytotoxicity in a dose‐dependent fashion. In addition, UV‐B exposure inhibited DNA and RNA synthesis. The role of non‐dimer DNA damage in the cytotoxicity induced by UV‐B is discussed.