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TIME‐RESOLVED FLUORESCENCE OF NITROBENZOXADIAZOLE‐AMINOHEXANOIC ACID: EFFECT OF INTERMOLECULAR HYDROGEN‐BONDING ON NON‐RADIATIVE DECAY
Author(s) -
Lin Su,
Struve Walter S.
Publication year - 1991
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1991.tb02028.x
Subject(s) - solvatochromism , chemistry , chromophore , photochemistry , hydrogen bond , fluorescence , intermolecular force , solvent , fluorophore , kinetic isotope effect , solvent effects , kinetics , iodide , deuterium , molecule , inorganic chemistry , organic chemistry , physics , quantum mechanics
The fluorescence kinetics of the nitrobenzoxadiazole (NBD) chromophore were studied at low concentrations in solvents with varying polarity and hydrogen‐bonding donor strength. The emission decay was essentially single exponential in all solvents studied. While the absorption and fluorescence solvatochromism is determined largely by the solvent polarity, the S, state decay kinetics are strongly modulated by the solvent H‐bonding capacity. The NBD emission lifetime, generally –7–10ns in the aprotic solvents, is reduced to 0.933 ns in water. The solvent deuterium isotope effect on the fluorescence decay is substantial in D 2 0 and in methanol‐d 4 , but is insignificant in DMSO‐d 6 . These results are consistent with acceleration of S 1 S 11 internal conversion through an accepting vibrational mode created by intermolecular hydrogen‐bonding of the NBD chromophore to an H atom‐donating solvent. This work bears on the practicality of using NBD as a fluorophore in assays for estrogen and progesterone receptors.