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SUBNANOSECOND SINGLE PHOTON TIMING MEASUREMENTS USING A PULSED DIODE‐LASER *
Author(s) -
Farrens David L.,
Song PillSoon
Publication year - 1991
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1991.tb02022.x
Subject(s) - laser , diode , photon counting , optoelectronics , materials science , photonics , fluorescence , laser diode , photon , optics , physics
The convenient and inexpensive use of a pulsed diode‐laser (Hamamatsu Photonics PLP‐01 660 nm) is demonstrated as a low cost alternative to a standard pulsed laser or gas discharge flash system in a commercial time‐correlated single photon counting instrument. Fluorescence lifetimes of compounds of photobiological interest such as phytochrome, chlorophyll a , 1,1 ′‐diethyl‐4,4′ carbocyanine iodide (DCI/cryptocyanin),5,10,15,20‐tetra(p‐phenyl) porphyrin and stentorin I are presented using the pulsed diode‐laser source.