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LOW TEMPERATURE POLARIZED ABSORPTION MICROSPECTROSCOPY OF SINGLE CRYSTALS OF THE REACTION CENTER FROM Rhodobacter sphaeroides WILD TYPE STRAIN 2.4.1
Author(s) -
Frank Harry A.,
Aldema Mila L.,
Violette Carol A.,
Parot Pierre H.
Publication year - 1991
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1991.tb02000.x
Subject(s) - rhodobacter sphaeroides , absorbance , photosynthetic reaction centre , absorption (acoustics) , strain (injury) , absorption spectroscopy , chemistry , analytical chemistry (journal) , photochemistry , materials science , optics , photosynthesis , organic chemistry , chromatography , medicine , biochemistry , physics , electron transfer , composite material
The absorbance and polarized absorbance spectra of single crystals of the reaction center complex isolated from Rhodobacter sphaeroides wild type strain 2.4.1 have been measured at 85 K. The crystals of the complex were obtained by the vapor diffusion technique. The spectroscopic experiments on the crystals were performed using an optical microspectrometer featuring a custom‐built, liquid N 2 ‐flowing cold stage, the details of which are presented herein. These data demonstrate the feasibility of conducting optical spectroscopic experiments at cryogenic temperatures on single crystals of photosynthetic pigment‐protein complexes.