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CELLULAR UPTAKE AND RELATIVE EFFICIENCY IN CELL INACTIVATION BY PHOTO ACTIVATED SULFONATED meso‐TETRAPHENYLPORPHINES
Author(s) -
Berg Kristian,
Bommer Jerry C.,
Winkelman James W.,
Moan Johan
Publication year - 1990
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1990.tb08681.x
Subject(s) - phototoxicity , fluorescence , sulfonate , chemistry , photochemistry , membrane , photosensitizer , biophysics , in vitro , optics , biochemistry , organic chemistry , physics , biology , sodium
— The cellular uptake, relative fluorescence quantum yields and photosensitizing efficiencies of meso‐tetraphenylporphines sulfonated to different degrees (TPPS n ) have been investigated using the human carcinoma cell line NHIK 3025. The efficiencies of these dyes in photoinactivation of cells were highly dependent on the number of sulfonate groups on the derivatives. These differences in phototoxicity were primarily due to different abilities to be taken up by cells, but were also dependent upon the cellular localization of the dyes. TPPS 1 and TPPS 2a were more efficiently taken up by the cells than TPPS 2o and TPPS 4 . Plasma membrane associated TPPS 4 was less efficient in cell inactivation per quantum of fluorescence emitted than intracellularly located dye. This was also to some extent the case for TPPS 1 but not for TPPS 2a and TPPS 2o . The results presented here indicate that TPPS 2a and TPPS 1 are the most promising of the TPPS n s for possible future use in photodynamic therapy.