Premium
THE HEMOLYSIS OF ERYTHROCYTES BY SINGLET OXYGEN GENERATED IN THE GAS PHASE
Author(s) -
Wang Tian P.,
Kagan Jacques,
Lee Sang,
Keiderling Timothy
Publication year - 1990
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1990.tb08677.x
Subject(s) - singlet oxygen , hemolysis , oxygen , chemistry , superoxide , gas phase , irradiation , photochemistry , ion , oxygen atom , phase (matter) , biophysics , biochemistry , immunology , molecule , biology , organic chemistry , physics , nuclear physics , enzyme
— Many sensitizers cause photodynamic hemolysis of erythrocytes. As these sensitizers usually participate in Type I as well as Type II processes, the determination of the mechanism(s) of photosensitized hemolysis is always ambiguous. Here, human erythrocytes were proved to hemolyze upon treatment with singlet oxygen ( 1 δ g ) generated with fiuoranthene in the gas phase. These conditions rigorously exclude the participation of superoxide anion. The standard diagnostic tests for singlet oxygen (enhanced effect in D 2 O and protection by NaN 3 ) gave the anticipated results when the erythrocytes were treated with 1 O 2 generated in the gas phase. When the erythrocytes were irradiated in a buffer solution containing fiuoranthene, the results of the diagnostic tests depended on the sensitizer concentration