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IS RHODAMINE 123 A PHOTOSENSITIZER?
Author(s) -
Morlière P.,
Santus R.,
Bazin M.,
Kohen E.,
Carillet V.,
Bon F.,
Rainasse J.,
Dubertret L.
Publication year - 1990
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1990.tb08670.x
Subject(s) - phototoxicity , photosensitizer , rhodamine 123 , singlet oxygen , chemistry , photosensitivity , rhodamine , mitochondrion , photochemistry , in vitro , cytochrome c , biophysics , fluorescence , oxygen , microbiology and biotechnology , biochemistry , biology , materials science , antibiotics , organic chemistry , optics , physics , optoelectronics , multiple drug resistance
— –Because of conflicting reports on the phototoxicity of rhodamine 123 (Rh 123), we have undertaken a study of Rh 123 photosensitization in several in vitro systems. First, Rh 123 is not a photodynamic agent and does not react with singlet oxygen. Second, when bound to cytochrome c (Cyt c), Rh 123 photosensitizes ferro Cyt c but not ferri Cyt c degradation by an oxygen‐independent process. When delivered to skin fibroblasts where it specifically stains mitochondria, Rh 123 photosensitizes membrane damage. These results are consistent with the hypothesis that Rh 123 is a phototoxic stain. The lack of photosensitivity of Rh 123‐stained mitochondria in some cell lines might therefore be due to specific structural features.