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SOME COMPONENTS OF THE TUMOR‐LOCALIZING FRACTION OF HEMATOPORPHYRIN DERIVATIVE
Author(s) -
Ho YauKwan,
Pandey Ravindra K.,
Missert Joseph R.,
Dougherty Thomas J.
Publication year - 1990
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1990.tb08449.x
Subject(s) - hematoporphyrin , chemistry , aluminum hydride , fraction (chemistry) , chromatography , photosensitizer , size exclusion chromatography , derivative (finance) , hydrolysis , ether , component (thermodynamics) , porphyrin , column chromatography , photodynamic therapy , biochemistry , organic chemistry , enzyme , catalysis , physics , methoxide , financial economics , economics , thermodynamics
— –In continuation of the effort to delineate the structure of Photofrin, a chromatographically well separated component of the tumor‐localizing fraction was isolated and purified using a combination of gel filtration chromatography and semi‐preparative high‐performance liquid chromatography. This component, the least hydrophobic of the tumor‐localizing fraction, was deemed to be dihematoporphy‐rin ether, based on mass spectrometric analysis and its behavior toward base hydrolysis and lithium aluminum hydride reduction. Although less potent than Photofrin, the purified component was an active photosensitizer.