Premium
THE USE OF FLUORESCENT PROBES IN IMMUNOCHEMISTRY *
Author(s) -
Davidson R. Stephen,
Hilchenbach Martin M.
Publication year - 1990
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1990.tb04200.x
Subject(s) - labelling , immunochemistry , fluorescence , fluorescent labelling , chemistry , computational biology , biochemistry , biology , antibody , immunology , optics , physics
— The limitations and advantages of particular dyes for labelling proteins and other biological materials are discussed. Methods available for conjugating dyes to proteins are outlined. Following a discussion of double labelling methods the use of photoactivatable fluorochromes and time resolved fluorescence methodologies are outlined. The reasons for the photoinstability of some fluorochromes are discussed and methods for overcoming the problem are described.