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CHARACTERIZATION OF RADICAL ADDUCTS FORMED DURING PHOTOCHEMICAL SPIN TRAPPING IN LIPOSOMES
Author(s) -
Sheng PeiGEN,
Feix J.,
Kalyanaraman B.
Publication year - 1990
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1990.tb04188.x
Subject(s) - chemistry , spin trapping , photochemistry , benzophenone , liposome , radical , photosensitizer , hydrogen peroxide , adduct , membrane , phospholipid , photodissociation , quenching (fluorescence) , organic chemistry , fluorescence , biochemistry , physics , quantum mechanics
— There exists an urgent need to monitor radical reactions in biological membranes. With the exception of a few studies in model and natural membranes, most biological spin trapping reactions have been confined to homogeneous media. In the present work we have devised a methodology by which spin trapping reactions can be investigated in liposomes composed of a fully‐saturated phospholipid, dimyristoylphosphatidylcholine. Using photochemical spin trapping, we have detected and characterized the formation and partitioning of spin adducts in liposomes. Benzophenone (a lipid‐soluble photosensitizer) and benzoyl peroxide (a lipid‐soluble radical initiator) have been used to generate free radicals from hydrogen donors during photolysis in liposomes. Both water‐soluble and lipid‐soluble spin traps have been used to trap the radicals.