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INVESTIGATIONS ON THE FLUORESCENCE CONCENTRATION QUENCHING OF FLAVOMONONUCLEOTIDE IN GLYCERINE‐WATER SOLUTIONS
Author(s) -
Grajek H.,
Zurkowska G.,
Bojarski C.,
Drabent R.
Publication year - 1990
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1990.tb01966.x
Subject(s) - quenching (fluorescence) , quantum yield , monomer , fluorescence , energy transfer , photochemistry , chemistry , yield (engineering) , radiative transfer , radiant energy , analytical chemistry (journal) , chemical physics , thermodynamics , polymer , chromatography , optics , physics , organic chemistry , radiation
We measured concentration changes of the fluorescence quantum yield η/η o of flavomononucleotide (FMN) in glycerine‐water solutions of various viscosities. The results obtained show that FMN dimers are traps for the exciting light energy as well as the energy transferred non‐radiatively. Very good agreement between the experimental and theoretical η/η o results was obtained. It has been shown that in the investigated solutions non‐radiative energy transfer from monomers to dimers takes place, preceded by the migration of energy. It has been stated that no monomer quenching occurs in these solutions. The contribution of the individual fluorescence concentration quenching mechanisms has been determined.